5 PRIME is a company founded in 2006 by the entrepreneurial spirit of a few life scientists. They are a powerful team with strong experience in the life science research market.

They provide molecular biology products and solutions for everyday laboratory research. Convenience, ease-of-use and time savings…their mission is to make your life easier in the laboratory. They offer a comprehensive range of products which include our own internally developed products, OEM products and the Eppendorf 5 Prime products acquired from a buy-out.

Please visit 5 Primes website at www.5prime.com

FastPlasmid Mini Kit

Feature and Benefits

• Enormous time savings with fast procedure
  
• One-step lysis for simplified handling
  
• Yields reproducible high quality plasmid DNA
  
• 1.5mL of bacterial culture yields up to 20µg

• Sequencing
  
• Transformation
  
• Cloning

Perfectprep Plasmid 96 Vac Kit

Feature and Benefits

• Rapid, high-throughput protocol-reduced number of steps & short incubations
  
• Highest purity: RNA and chromosomal DNA free
  
• Reproducible elution for consistent plasmid concentrations

Applications

• Sequencing
  
• Transformation
  
• Transfection
  
• PCR
  

Perfectprep BAC 96 Kit

Feature and Benefits

• 96 samples processed in 60 minutes
  
• Unique technology results in minimal DNA shearing
  
• Sufficient yield for four sequencing reactions and one fingerprint

Applications

• PCR
  
• Sequencing
  
• Fingerprinting
  
• Transformation

GelElute Extraction Kit

• Feature and Benefits
  
• Convenient extraction of DNA from 40bp to 50kb
  
• Gel extraction from agarose and polyacrylamide gels
  
• No sodium iodide to interfere with subsequent reactions

Applications

• Restriction Digestion
  
• Labeling
  
• Ligation
  
• PCR

ArchivePure is a highly flexible and scalable method for the isolation of archivable quality genomic DNA for a multitude of sample types without the use of harmful chemicals.

Feature and Benefits

• DNA purification from a wide variety of sample types including blood, buffy coat, cultured cells and tissue with a single kit
  
• Premixed and ready-to-use
  
• solates high molecular weight DNA with exceptional purity

Applications

• PCR & Real-time PCR
  
• Southern Blotting
  
• Sequencing
  
• SNP Analysis
  
• Archiving

PerfectPure DNA Purification KITS

PerfectPure DNA kits are a novel column-based technology that eliminates the most common problems of genomic DNA purification such as overloading and clogging.

Feature and Benefits

• Straightforward and efficient process
  
• High quality genomic DNA from whole blood and cultured cells
  
• Non-toxic procedure
  
• enomic DNA free from contaminants

Applications

• DNA Microarray
  
• PCR & Real-time PCR
  
• Southern Blotting

PerfectPure RNA Purification KITS

PerfectPure RNA kits are a novel column-based technology that eliminates the most common problems of RNA purification such as overloading and clogging.

Feature and Benefits

• New column-based technology
  
• Fast and efficient process
  
• Stable, high quality RNA, free of genomic DNA, proteins and other enzymatic inhibitors from whole blood, cultured cells and tissue.
  
• Complete kit, ready-to-use out of the box

Applications

• Microarray
  
• Real-time PCR
  
• RT-PCR

Ready PCR kits employ a unique filtration method which consistently produces high yields of PCR-ready DNA for screening.

Feature and Benefits

• Simple, two solution system
  
• Quick and easy protocol
  
• Provides PCR-ready genomic DNA for quick screening
  
• Yields up to 60µg per sample

Applications

• PCR

Feature and Benefits

• Minimal PCR optimization required
  
• High yield and specificity

Feature and Benefits

• Ready-to-use master mix format
  
• Minimal optimization with self-adjusting Mg2+ buffer technology
  
• Convenient 4°C storage eliminates freeze and thaw cycles

Applications

• Standard PCR
  
• High-throughput PCR

Feature and Benefits

• Improved Taq DNA Polymerase processivity with TaqMaster PCR Enhancer
  
• Extremely robust amplification with high yields
  
• High tolerance of PCR inhibitors

Applications

• Amplification of GC-rich targets
  
• Amplification of difficult samples like soil and mouse tail

Feature and Benefits

• Highly specific amplification
  
• Minimal optimization with self-adjusting Mg2+ buffer technology
  
• No enzyme activation-excellent for fast PCR protocols
  
• Convenient reaction set-up at room temperature
• Applications
  
• Hot-Start PCR
  
• Amplification of complex templates

Feature and Benefits

• Highly specific PCR
  
• Ready-to-use master mix format
  
• Minimal optimization with self-adjusting Mg2+ buffer technology
  
• Convenient 4°C storage eliminates freeze and thaw cycles

Applications

• Hot-Start PCR
  
• Automated PCR setup
  
• High-throughput PCR

Feature and Benefits

• Efficient amplification up to 40kb with high yields
  
• Low error rate and optimized processivity with proofreading enzyme
  
• Minimal optimization with self-adjusting Mg2+ buffer technology
  
• Optimized amplification of complex or rare targets

Applications

• Long-range PCR
  
• High-fidelity PCR
  
• Amplification of difficult targets

Feature and Benefits

• Robust amplification with hot-start enzyme
  
• Accurate products by efficient proofreading enzyme
  
• Minimal PCR optimization due to special buffer formulation

Applications

• Cloning
  
• Site-directed Mutagenesis
  
• Mutation Analysis

Feature and Benefits

• Full-length cDNA fragments up to 12kb
  
• Highly sensitive reverse transcription from low RNA amounts
  
• Reverse transcription at high temperature
  
• Complete kit with Stop RNase Inhibitor and dNTP's

Applications

• First-strand cDNA synthesis
  
• Two-step RT-PCR
  
• Two-step real-time RT-PCR

Feature and Benefits

• High yield reverse transcription and optimized PCR reaction
  
• Proofreading PCR enzyme mix for long-range RT-PCR up to 12kb
  
• Minimal optimization with self-adjusting Mg2+ buffer technology
  
• Complete kit with Stop RNase Inhibitor and dNTP's

Applications

• One-step RT-PCR up to 6kb
  
• Two-step RT up to 12kb
  
• High-fidelity RT-PCR

Feature and Benefits

• Superior sensitivity and specificity with Hot-Start/Cold-Stop technology
  
• Special formulation to minimize primer-dimer formation
  
• Minimal optimization with self-adjusting Mg2+ buffer technology
  
• Easy set-up with ready-to-use master mix format
  
• Available with or without ROX for normalization

Applications

• Quantitative and qualitative real-time PCR
  
• Optimized for sequence-specific probes

Feature and Benefits

• Highly sensitive quantification with cost-effective SYBR detection
  
• Special formulation to minimize primer-dimer formation
  
• Minimal optimization with self-adjusting Mg2+ buffer technology
  
• Easy set-up with ready-to-use master mix format
  
• Contains ROX for normalization

Applications

• Quantitative and qualitative real-time PCR
  
• Optimized for SYBR Green

Deoxynucleotide Mix

Molecular Biology Grade Water, Cloning Reagents, Buffers, Enzymes and accessories

Norgen Biotek

Norgen Biotek Corporation is a highly innovative privately owned Canadian Biotechnology Company. Norgen has developed two platform technologies for the purification of proteins and nucleic acids. Recently, the company started to manufacture bio-consumable products (kits) addressing the needs of the genomic, proteomic and diagnostic markets.

ProteoSpin Protein Kits

• Six (6) different kits available for protein sample preparation
• All Column based chemistry
• CBED (Concentration, Buffer Exchange and Desalting) Kits concentrate dilute solutions of proteins, buffer exchange and desalt protein samples. Conventional salt removal techniques in protein samples are time consuming and these kits are fast and simple.
• Detergent Clean-Up kits remove any detergent in samples that will interfere with downstream applications.
• Inclusion Body Kits isolate and purify the recombinant proteins found in sub-cellular structures called Inclusion Bodies.
• Urine Protein Concentration Kits concentrate proteins in urine for identification of potential biomarkers in urine and to diagnose and/or monitor diseases.
• Abundant Serum Kits remove proteins present in body fluids in high concentrations (e.g. Albumin and y-globulin) which may mask detection and analysis of less abundant proteins.
• On-Column Proteolytic Kits rapidly enzymatically digest simple and complex proteins using Trypsin and then purify the resulting peptides.
• Kits can be used in any clinical, research or biopharmaceutical laboratory.

Click here for kit component data. See the chart below for product information and package inserts.

• Eight (8) different kits available for DNA sample preparation.
• All column based chemistry.
• DNA Gel Extraction Kit is for the rapid purification of DNA fragments from agarose gels.
• PCR Purification Kit purifies amplified DNA products from PCR mixes.
• Plasmid Kit provides a rapid analytical scale preparation of plasmid DNA.
• BAC DNA Kit is for the preparation of BAC's and other large DNA constructs from small batches of E.Coli.
• Urine DNA Kit isolates DNA from 2mL of urine.
• Bacterial Genomic DNA Kit isolates genomic DNA from 2 x 109 viable cells.
• Yeast Genomic DNA Kit isolates genomic DNA from 1 x 108 viable yeast cells.
• Blood Genomic DNA Kit isolates genomic DNA from up to 100µL of blood

• Column based chemistry for the rapid isolation and purification of RNA from cultured animal cells, bacterial cells, blood and small tissue samples.

Click here for kit component data. See the chart below for product information and package inserts.

• Seventeen (17) DNA molecular weight ladders are available in a variety of different sizes and fragment ranges.
• Two (2) RNA ladders are available and three (3) Protein ladders are available.