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T and B Cell Clonality Testing – Cell Line Controls

Tags: Molecular
Controls are available in several different formats: plasmid DNA, DNA extracted from tissue or cell lines, or RNA extracted from cell lines. These controls can be purchased in various dilutions or as complete dilution sets and panels for several purposes, such as in the development of laboratory-developed tests (LDTs), assay validation, sensitivity or proficiency testing, and troubleshooting.

Cell Line DNA and RNA controls are extracted from established cell lines grown under cell culture conditions recommended by the supplier. IVS controls are tested extensively to ensure quality and reproducibility of your test results. Please note, these controls are for qualitative use only.

The DNA controls, including sensitivity controls, are supplied in aliquots or 100 µL and are adjusted to a final concentration of 200 µg/mL in 1/10 TE (1 mM Tris-HCl (pH 8.0), 0.1 mM EDTA). This diluent provides sufficient buffering capacity and EDTA to protect the DNA controls without interfering with the Mg+2 concentrations required for robust amplification reactions. DNA dilutions are diluted volume to volume in IVS negative control DNA, IVS-0000 Polyclonal DNA.

The RNA controls, including sensitivity controls, are supplied in aliquots or 100 µL and are adjusted to a final concentration of 400 µg/mL in RNase-free glass-distilled water. The pH of distilled water is slightly acidic; this protects the RNA from hydrolysis.

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