Pathology

Immunohistochemistry detection kits and chromogens

Tags: Pathology
The MACH 1 Universal Polymer Detection system offers a cost-effective alternative method of polymer detection for IHC laboratories (not available in the U.S.). Specifically titered for Biocare prediluted antibodies, MACH 1 provides ­superior staining and a reduced protocol time as compared to the conventional ABC ­system.

MACH 1

The MACH 1 Universal Polymer Detection system offers a cost-effective alternative method of polymer detection for IHC laboratories (not available in the U.S.). Specifically titered for Biocare prediluted antibodies, MACH 1 provides ­superior staining and a reduced protocol time as compared to the conventional ABC ­system.

This new two-step, biotin-free technology uses a specific probe to detect mouse antibodies. The probe is followed by an HRP-polymer that binds to both the probe and to rabbit antibodies. Time consuming avidin-biotin and normal serum blocking steps are eliminated. The innovative HRP-polymer technology provides superior staining for mouse and rabbit antibodies when compared to ABC, biotin-streptavidin (BSA) and other polymer detection kits.

MACH 1 Features:
– Elimination of avidin-biotin and normal serum blocking steps
– Reduce protocol length by approximately 90 minutes compared to ABC method
– High value, cost-effective alternative to biotin-streptavidin or ABC detection
– Eliminates endogenous non-specific background staining

Catalgue Number:
– BC-M1U539G (6 mL)
– BC-M1U539L10 (110 mL)

MACH 2

MACH 2 Detection is a one-step / one-solution, biotin-free detection system which combines superior work flow and sensitivity for mouse and rabbit primary antibodies.

MACH 2 detection consists of a single reagent applied after the primary antibody. MACH 2 is ideal for use with prediluted antibodies or concentrates with equal success. MACH 2 may be used for 7-step multiplex stains with certain antibodies.

MACH 2 increases staining specificity compared to conventional dextran backbone polymer. MACH 2 is available in anti-mouse, anti-rabbit, and ‘universal’ (anti-mouse & anti-rabbit) formulations, labeled with either Horseradish Peroxidase (HRP) or Alkaline Phosphatase (AP).

MACH 2 Features:
– Increase specificity 3-4 fold in nuclear staining
– Increase specificity 2 fold in cytoplasmic/cell surface staining
– Superior to dextran backbone polymers

Catalogue Number:
– BC-MALP521G (6 mL); BC-MALP521H (25 mL); BC-MALP521L (100 mL)
– BC-RALP525G (6 mL), BC-RALP525H (25 mL), BC-RALP525L (100 mL)
– BC-MHRP520G (6 mL), BC-MHRP520H (25 mL), BC-MHRP520L (100 mL)
– BC-RHRP520G (6 mL), BC-RHRP520H (25 mL), BC-RHRP520L (100 mL)
– BC-M2U522G (6 mL), BC-M2U522H (25 mL), BC-M2U522L (100 mL)

MACH 3

MACH 3 is a two-step, biotin-free detection system which provides excellent specificity, sensitivity and nuclear staining for mouse or rabbit primary antibodies.

MACH 3 Features:
– A sensitivity-enhancing secondary reagent
– Increased density of enzymes bound to tertiary target
– 5- to 10- fold increased staining sensitivity when compared to conventional dextran polymer detection kits
– Superior staining for nuclear and cytoplasmic/cell surface antigens
– Elimination of background
– High primary antibody dilution significantly improves specificity and reduces cost
– Adapted for all Biocare’s prediluted antibodies
– Compatible with automated immunostainers

Catalogue Number:
– BC-M3M532G (6 mL), BC-M3M532H (25 mL), BC-M3M532L (100mL)
– BC-M3M530G (6 mL), BC-M3M530H (25 mL), BC-M3M530L (100mL)
– BC-M3R533G (6 mL), BC-M3R533H (25 mL), BC-M3R533L (100mL)
– BC-M3R531G (6 mL), BC-M3R531H (25 mL), BC-M3R531L (100mL)

MACH 4

MACH 4 is a highly sensitive universal detection system. The MACH 4 system can detect either mouse or rabbit antibodies, and is a two-step universal detection method. MACH 4 micro-polymer system incorporates two key technologies:
– Enhanced probe to increase sensitivity 10- to 20- fold
– Increased density of enzymes bound to tertiaries

MACH 4 Features:
– 10 – 20 times more sensitive than conventional dextran polymer systems
– 20 – 40 times more sensitive for nuclear staining
– Suitable to detect mouse specific anti-biotin or antidigoxigenin antibody in in situ hybridization (ISH)
– Micro-polymer allows superior specificity/sensitivity, nuclear access and primary antibody dilution
– Compatible with automated immunostainers

Catalogue Numbers;
– BC-BRI4012G (6 mL), BC-BRI4012H (25 mL), BC-BRI4012L (100 mL) – Complete Kits (Detection, Block and DAB Chromogen)
– BC-BRI4005L (100 mL) – Complete Kits (Detection, Block and Fast Red Chromogen)
– BC-M4U536G (6 mL), BC-M4U536H (25 mL), BC-M4U536L (100 mL)
– BC-M4U534G (6 mL), BC-M4U534H (25 mL), BC-M4U534L (100 mL)

Chromogens

Biocare Medical offers the largest variety of IHC-specific permanent chromogens, for use with the two most commonly used enzyme labels: Horseradish Peroxidase (HRP) and Alkaline Phosphatase (AP). Chromogens are chemical compounds used within IHC staining procedures that produce a coloured end-product that can be visualized under bright-field microscopy. The chromogens generate a broad spectrum of colours, shown below.

Chromogens for HRP; End-Product Colour
– Betazoid DAB (3rd generation); Brown
– Cardassian DAB (2nd generation); Black Brown
– DAB (1st generation); Brown
– Romulin AEC; Brick Red
– Bajoran Purple; Purple
– Vina Green; Green
– Deep Space Black™; Black

Chromogens for AP; End-Product Colour
– Warp Red™; Fuchsin Red
– Vulcan Fast Red (VFR); Fuchsin Red
– Ferangi Blue; Royal Blue “

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